Authors: Leandro Rivel, Camila; Jay-Robert, Pierre;Pétillon, Julien;

Arthropods is by far the phylum that has the most species and the most individuals-rich of all the animal kingdom. Nevertheless, their effectives have dramatically decreased these last decades. Due to technical difficulties to survey and relatively few taxonomists, terrestrial arthropods have drawn little attention in the field of environmental DNA (eDNA). Nonetheless, some pioneer studies and approaches have shown the many promises of arthropod eDNA surveys per se or to achieve terrestrial species distributions knowledge gathering through arthropods. Such methods have bypassed some of the inherent difficulties of eDNA surveys in non-aquatic environments by creative approaches. They have also pointed to difficulties and bias, such as the lack of completeness of barcode libraries that we assessed for spiders, springtails and some insect orders. In addition, such techniques not only address data acquisition and methodological questions but also have the potential to address management and conservation questions. In this systematic review of the literature, we acknowledge and discuss the models, methods and aims of the available literature on eDNA targeting terrestrial arthropods. We also assed arthropods' barcode coverage by public repositories of genetic data. Moreover, we illustrate a step-by-step procedure with data from a program conducted in French national parks.